Generator

Part:BBa_K1820020

Designed by: Sara Gertsch   Group: iGEM15_Utah_State   (2015-09-16)

P335-Φ31 inducible middle promoter + RBS+ sfGFP + term

A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31 with the B0034 ribosomal binding site (RBS) (BBa_K1820001), a super-folded green fluorescent protein (sfGFP) from the 2014 Groningen team that was codon optimized for Bacillus subtilis (BBa_K1365020), and a transcriptional terminator.

Usage and Biology

This construct was designed to initiate transcription after phage infection of Lactococcus lactis, resulting in expression of sfGFP (previously shown to have a high level of fluorescent activity in L. lactis). Allowing for fluorescent detection of Φ31 phage infection in L. lactis.

This construct has yet to be cloned and expressed in L. lactis, and validated for functionality.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 42
    Illegal SapI.rc site found at 284


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Parameters
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